13 November 2014 Remote excitation fluorescence correlation spectroscopy using silver nanowires
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Abstract
Fluorescence correlation spectroscopy (FCS), a powerful tool to resolve local properties, dynamical process of molecules, rotational and translational diffusion motions, relies on the fluctuations of florescence observables in the observation volume. In the case of rare transition events or small dynamical fluctuations, FCS requires few molecules or even single molecules in the observation volume at a time to minimize the background signals. Metal nanoparticle which possess unique localized surface plasmon resonance (LSPR) have been used to reduce the observation volume down to sub-diffraction limited scale while maintain at high analyst concentration up to tens of micromolar. Nevertheless, the applications of functionalized nanoparticles in living cell are limited due to the continuous diffusion after cell uptake, which makes it difficult to target the region of interests in the cell. In this work, we demonstrate the use of silver nanowires for remote excitation FCS on fluorescent molecules in solution. By using propagation surface plasmon polaritons (SPPs) which supported by the silver nanowire to excite the fluorescence, both illumination and observation volume can be reduced simultaneously. In such a way, less perturbation is induced to the target region, and this will broaden the application scope of silver nanowire as tip in single cell endoscopy.
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Liang Su, Haifeng Yuan, Gang Lu, Johan Hofkens, Maarten Roeffaers, Hiroshi Uji-i, "Remote excitation fluorescence correlation spectroscopy using silver nanowires", Proc. SPIE 9277, Nanophotonics and Micro/Nano Optics II, 92771A (13 November 2014); doi: 10.1117/12.2071376; https://doi.org/10.1117/12.2071376
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