A versatile fluorescence lifetime imaging system for scanning large areas with high time and spatial resolution

César Bernardo; Michael Belsley; Etelvina de Matos Gomes; Hugo Gonçalves; Dmitry Isakov; Falk Liebold; Eduardo Pereira; Vladimiro Pires; Anura Samantilleke; Mikhail Vasilevskiy; Peter Schellenberg

doi:10.1117/12.2063940

22 August 2014 A versatile fluorescence lifetime imaging system for scanning large areas with high time and spatial resolution

César Bernardo, Michael Belsley, Etelvina de Matos Gomes, Hugo Gonçalves, Dmitry Isakov, Falk Liebold, Eduardo Pereira, Vladimiro Pires, Anura Samantilleke, Mikhail Vasilevskiy, Peter Schellenberg

Author Affiliations +

Proceedings Volume 9286, Second International Conference on Applications of Optics and Photonics; 928637 (2014) https://doi.org/10.1117/12.2063940
Event: Second International Conference on Applications of Optics and Photonics, 2014, Aveiro, Portugal

Abstract

We present a flexible fluorescence lifetime imaging device which can be employed to scan large sample areas with a spatial resolution adjustable from many micrometers down to sub-micrometers and a temporal resolution of 20 picoseconds. Several different applications of the system will be presented including protein microarrays analysis, the scanning of historical samples, evaluation of solar cell surfaces and nanocrystalline organic crystals embedded in electrospun polymeric nanofibers. Energy transfer processes within semiconductor quantum dot superstructures as well as between dye probes and graphene layers were also investigated.

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César Bernardo, Michael Belsley, Etelvina de Matos Gomes, Hugo Gonçalves, Dmitry Isakov, Falk Liebold, Eduardo Pereira, Vladimiro Pires, Anura Samantilleke, Mikhail Vasilevskiy, and Peter Schellenberg "A versatile fluorescence lifetime imaging system for scanning large areas with high time and spatial resolution", Proc. SPIE 9286, Second International Conference on Applications of Optics and Photonics, 928637 (22 August 2014); https://doi.org/10.1117/12.2063940

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