25 November 2014 Genomes correction and assembling: present methods and tools
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Proceedings Volume 9290, Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2014; 92901X (2014) https://doi.org/10.1117/12.2075624
Event: Symposium on Photonics Applications in Astronomy, Communications, Industry and High-Energy Physics Experiments, 2014, Warsaw, Poland
Abstract
Recent rapid development of next generation sequencing (NGS) technologies provided significant impact into genomics field of study enabling implementation of many de novo sequencing projects of new species which was previously confined by technological costs. Along with advancement of NGS there was need for adjustment in assembly programs. New algorithms must cope with massive amounts of data computation in reasonable time limits and processing power and hardware is also an important factor. In this paper, we address the issue of assembly pipeline for de novo genome assembly provided by programs presently available for scientist both as commercial and as open – source software. The implementation of four different approaches – Greedy, Overlap – Layout – Consensus (OLC), De Bruijn and Integrated resulting in variation of performance is the main focus of our discussion with additional insight into issue of short and long reads correction.
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Michał Wojcieszek, Michał Wojcieszek, Magdalena Pawełkowicz, Magdalena Pawełkowicz, Robert Nowak, Robert Nowak, Zbigniew Przybecki, Zbigniew Przybecki, } "Genomes correction and assembling: present methods and tools", Proc. SPIE 9290, Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2014, 92901X (25 November 2014); doi: 10.1117/12.2075624; https://doi.org/10.1117/12.2075624
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