The properties of energy conditioning electrical circuits that are developed for powering additional functional blocks of autonomous RFID transponders working in the HF band have been analyzed and presented in the paper. The concept of autonomy is realized by implementing extra functions in the typical transponder. First of all, the autonomous system should harvest energy, e.g. from the electromagnetic field of read/write devices but also the possibility of gathering information about environment should be available, e.g. by measuring different kind of physical quantities. In such an electrical device, the crucial problem consists in energy conditioning because the output voltage-current characteristic of an front-end (antenna with matching and harvesting circuit) as well as the total and instantaneous power load generated by internal circuits are strongly dependent on a realized function but also on energy and communication conditions in the RFID interface. The properly designed solution should improve harvesting efficiency, current leakage of supply storage, matching between antenna and input circuits, in order to save energy and increase operating time in such a battery-free system. The authors present methods how to increase the autonomous operation time even at advanced measuring algorithms. The measuring system with wide spectrum of sensors dedicated for different quantities (physical, chemical, etc.) has also been presented. The results of model calculations and experimental verifications have been also discussed on the basis of investigations conducted in the unique laboratory stand of object navigation systems.