Development of a rapid optic bacteria detecting system based on ATP bioluminescence

Jun Tao Liu; JinPing Luo; XiaoHong Liu; XinXia Cai

doi:10.1117/12.2069489

3 December 2014 Development of a rapid optic bacteria detecting system based on ATP bioluminescence

Jun Tao Liu, JinPing Luo, XiaoHong Liu, XinXia Cai

Proceedings Volume 9297, International Symposium on Optoelectronic Technology and Application 2014: Laser and Optical Measurement Technology; and Fiber Optic Sensors; 929706 (2014) https://doi.org/10.1117/12.2069489
Event: International Symposium on Optoelectronic Technology and Application 2014, 2014, Beijing, China

Abstract

A rapid optic bacteria detecting system based on the principle of Adenosine triphosphate(ATP) bioluminescence was presented in this paper. This system consisted of bioluminescence-based biosensor and the high-sensitivity optic meter. A photon counting photomultiplier tube (PMT) module was used to improve the detection sensitivity, and a NIOS II/f processor based on a Field Programmable Gate Array(FPGA) was used to control the system. In this work, Micrococcus luteus were chosen as the test sample. Several Micrococcus luteus suspension with different concentration was tested by both T2011 and plate counting method. By comparing the two group results, an calibration curve was obtained from the bioluminescence intensity for Micrococcus luteus in the range of 2.3×10² ~ 2.3×10⁶ CFU/mL with a good correlation coefficient of 0.960. An impacting Air microorganism sampler was used to capture Airborne Bacteria, and 8 samples were collected in different place. The TBC results of 8 samples by T2011 were between 10 ~ 2×10³ cfu/mL, consistent with that of plate counting method, which indicated that 8 samples were between 10 ~ 3×10³ cfu/mL. For total airborne bacteria count was small, correlation coefficient was poor. Also no significant difference was found between T2011 and plate counting method by statistical analyses.

Citation Download Citation

Jun Tao Liu, JinPing Luo, XiaoHong Liu, and XinXia Cai "Development of a rapid optic bacteria detecting system based on ATP bioluminescence ", Proc. SPIE 9297, International Symposium on Optoelectronic Technology and Application 2014: Laser and Optical Measurement Technology; and Fiber Optic Sensors, 929706 (3 December 2014); https://doi.org/10.1117/12.2069489

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