An experimental model for minimizing errors in laser speckle contrast imaging for microcirculation analysis

N. Sujatha; Arnab Banerjee

doi:10.1117/12.2077477

4 March 2015 An experimental model for minimizing errors in laser speckle contrast imaging for microcirculation analysis

N. Sujatha, Arnab Banerjee

Author Affiliations +

Proceedings Volume 9302, International Conference on Experimental Mechanics 2014; 93021R (2015) https://doi.org/10.1117/12.2077477
Event: International Conference on Experimental Mechanics 2014, 2014, Singapore, Singapore

Abstract

Understanding the changes in microcirculatory flow and its measurements are very important for assessing the progress of various vascular malfunctions and their subsequent treatment effectiveness. Laser Speckle Contrast Imaging (LSCI) has been evolved as a whole-field, non-invasive and non-contact technique which has inherent advantages for microcirculation assessment in an in vivo environment compared to its noninvasive counterparts such as laser Doppler technique and video capillaroscopy. However, representation of flow velocity values in absolute units is still challenging and yet to be completely explored. In this paper, we propose an experimental model for estimating the flow velocity based for optimum camera exposure time. The LSCI experiments were conducted on a custom made phantom flow channel with induced flow in the microcirculation range using a syringe pump. The speckle image contrast was estimated temporally and is used to calculate velocity values. The relative error in the flow values is estimated to be a function of the calculated contrast. The estimated error has been incorporated as a correction factor in the obtained velocity term using LSCI and final velocity estimation was found to be within an acceptable error range independent of the flow velocity and scatterer concentration of the sample for optimum camera exposure duration.

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N. Sujatha and Arnab Banerjee "An experimental model for minimizing errors in laser speckle contrast imaging for microcirculation analysis", Proc. SPIE 9302, International Conference on Experimental Mechanics 2014, 93021R (4 March 2015); https://doi.org/10.1117/12.2077477

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