9 April 2015 Front Matter: Volume 9305
This PDF file contains the front matter associated with SPIE Proceedings Volume 9305, including the Title Page, Copyright information, Table of Contents, Invited Panel Discussion, and Conference Committee listing.

The papers included in this volume were part of the technical conference cited on the cover and title page. Papers were selected and subject to review by the editors and conference program committee. Some conference presentations may not be available for publication. The papers published in these proceedings reflect the work and thoughts of the authors and are published herein as submitted. The publisher is not responsible for the validity of the information or for any outcomes resulting from reliance thereon.

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Author(s), "Title of Paper," in Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II, edited by Henry Hirschberg, Steen J. Madsen, E. Duco Jansen, Qingming Luo, Samarendra K. Mohanty, Nitish V. Thakor, Proceedings of SPIE Vol. 9305 (SPIE, Bellingham, WA, 2015) Article CID Number.

ISSN: 1605-7422

ISBN: 9781628413953

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Numbers in the index correspond to the last two digits of the six-digit citation identifier (CID) article numbering system used in Proceedings of SPIE. The first four digits reflect the volume number. Base 36 numbering is employed for the last two digits and indicates the order of articles within the volume. Numbers start with 00, 01, 02, 03, 04, 05, 06, 07, 08, 09, 0A, 0B...0Z, followed by 10-1Z, 20-2Z, etc.

Abdurashitov, A. S., 1D

Abookasis, David, 0L

Agus, Viviana, 2T

Alarcon, Joseph, 0U

Alexandrakis, George, 0N, 1A

Allegra Mascaro, Anna Letizia, 14, 1O

Andrásfalvy, Bertalan K., 0Y

Angelo, Joseph, 0J

Barbic, Mladen, 0Y

Bellow, S., 2Y

Berg, Kristian, 0C

Berndt, D., 2Y

Brideau, Craig, 04

Cao, Jianwei, 0N

Chen, Min, 2I

Chen, Wei, 0Z, 17

Choi, Chulhee, 1Y

Choi, Jeonghun, 0Z

Christie, Catherine E., 0C

Clegg, Nancy J., 0N

Costantini, Irene, 14, 1O

De Vittorio, Massimo, 2O

Delehanty, James B., 0Y

Delgado, Mauricio R., 0N

Di Giovanna, Antonino Paolo, 14

Di Silvio, Alberto, 2T

Diaz-Botia, Camilo, 2K

Dorian, Paul, 1G

Du, Congwu, 0Z, 17

Faraon, Andrei, 2L

Farooq, Hamza, 0Q, 0U

Field, Lauren D., 0Y

Fowler, Trevor, 2L

Gad, Raanan, 2H

Galiñanes, Gregorio L., 0Y

Gao, Feng, 1X

Gao, Yuan, 2I

Genis, Helen, 0Q

Geurts, Jeroen J., 04

Gioux, Sylvain, 0J

Gong, Hui, 27

Gu, Xijia, 05

Hanson, Tim, 2K

Heber, J., 2Y

Hervey, Nathan, 0N, 1A

Hetian, Yiyi, 2I

Hirschberg, Henry, 0C, 0E

Hodics, Timea, 1A

Hosokawa, Chie, 2N

Hososhima, Shoko, 2R

Hu, Xudong, 1G

Huang, Yize, 05, 0A

Huber, Daniel, 0Y

Huston, Alan L., 0Y

Ishizuka, Toru, 2R

Jakubovic, Raphael, 0U

Jia, Mengyu, 1X

Jiang, Tianzi, 1B

Jivraj, Jamil, 05, 07, 0A, 0Q

Kajbeleva, E. I., 1D

Kassim, M., 1D

Kawasoe, Jean H., 04

Kawauchi, Satoko, 0M, 23, 26

Khan, Bilal, 0N, 1A

Khan, Osaama H., 07

Kim, Siwook, 1G

Kiviniemi, Vesa, 1S

Klauser, Antoine M., 04

Klaver, Roel, 04

Koletar, Margaret, 2H

Kondraske, George, 1A

Korhonen, Vesa, 1S

Kudoh, Suguru N., 2N

Kwon, Young J., 0C

Ledochowitsch, Peter, 2K

Lee, Seunghee, 1Y

Lejeune, Jean-Paul, 0D

Leroy, Henri-Arthur, 0D

Levi, Ofer, 2H

Li, James, 17

Li, Ting, 2I

Lin, Michael Z., 34

Liu, Hanli, 0N

Liu, Ming, 1X

Liu, Peng, 17

Lohmer, Stefan, 2T

Lu, Yi, 05

Luo, Qingming, 27

Lychagov, V. V., 1D

MacFarlane, Duncan, 0N

Macklin, John J., 0Y

Madsen, Steen J., 0C, 0E

Maharabiz, Michel M., 2K

Makara, Judit K., 0Y

Mathews, Marlon S., 0L

Medintz, Igor L., 0Y

Miyaki, Mai, 26

Mondini, Anna, 2T

Montag, Katharina, 2T

Mordon, Serge, 0D

Müllenbroich, Marie Caroline, 14

Myllylä, Teemu, 1S

Nawashiro, Hiroshi, 26

Nishidate, Izumi, 0M, 26

Nosrati, Reyhaneh, 1G

Okuda, Wataru, 26

Pan, Yingtian, 0Z, 17

Park, Kicheon, 0Z

Pavlov, A.N., 1D

Pavone, Francesco S., 14, 1O

Pisanello, Ferruccio, 2O

Pisanello, Marco, 2O

Poon, Kelvin W., 04

Qi, Yisong, 27

Qian, Jun, 1J

Qin, Zhuanping, 1X

Ramadeen, Andrew, 1G

Ramjist, Joel, 05, 0A, 0Q

Redaelli, Loredana, 2T

Reid, Dahlia, 0N

Reyns, Nicolas, 0D

Ringuette, Dene, 2H

Roberts, Heather, 0N

Rolland, Jean Francois, 2T

Roukes, Michael L., 2L

Rückerl, F., 2Y

Sabes, Philip N., 2K

Sacconi, Leonardo, 14, 1O

Sato, Manabu, 0M

Sato, Shunichi, 0M, 23, 26

Scarabottolo, Lia, 2T

Schenk, Geert J., 04

Segev, Eran, 2L

Semyachkina-Glushkovskaya, O. V., 1D

Shagman, Laura, 0N

Shierk, Angela, 0N

Shih, En-Chung, 0E

Shorte, S., 2Y

Si, Juanning, 1B

Siegler, Peter, 07

Sigal, Iliya, 2H

Sileo, Leonardo, 2O

Silvestri, Ludovico, 14, 1O

Sindeev, S. S., 1D

Sindeeva, O. V., 1D

Smith, Linsley, 0N

Stefanovic, Bojana, 2H

Stowe, Ann, 1A

Stys, Peter K., 04

Su, Yu, 2I

Sun, Cuiru, 07, 0A, 0Q

Sun, Yunlong, 2I

Susumu, Kimihiro, 0Y

Taguchi, Takahisa, 2N

Takeda, Naoko, 2N

Takemura, Toshiya, 23, 26

Terakawa, Mitsuhiro, 23

Tétard, Marie-Charlotte, 0D

Tian, Fenghua, 0N

Tinevez, J-Y., 2Y

Toronov, Vladislav, 1G

Tremolada, Sara, 2T

Tsunoi, Yasuyuki, 23

Tuchin, Valery, 1D, 1S

Tulchin-Francis, Kirsten, 0N

Valdes, Pablo A., 0J

Vermandel, Maximilien, 0D

Volkov, Boris, 0L

Vuong, Barry, 05, 0A, 0Q

Wagner, M., 2Y

Watanabe, Ryota, 23

Woldemichael, Ermias, 1G

Wong, Ronnie J., 05, 07, 0A, 0Q

Yang, Victor X. D., 05, 07, 0A, 0Q, 0U

Yawo, Hiromu, 2R

Yazdan-Shahmorad, Azadeh, 2K

Yoon, Jonghee, 1Y

Yoshida, Keiichiro, 0M

Yu, Tingting, 27

Yuasa, Hideya, 2R

Zamora, Genesis, 0C

Zhang, Xin, 1B

Zhang, Yujin, 1B

Zhao, Huijuan, 1X

Zhao, Ruirui, 1B

Zhao, Yue, 2I

Zhou, Xin X., 34

Zhu, Dan, 27

Zinchenko, E. M., 1D

Zuo, Nianming, 1B

Conference Committee

  • Symposium Chairs

    • James G. Fujimoto, Massachusetts Institute of Technology (United States)

    • R. Rox Anderson, Wellman Center for Photomedicine, Massachusetts General Hospital (United States) and Harvard School of Medicine (United States)

  • Program Track Chair

    • Brian Jet-Fei Wong, Beckman Laser Institute and Medical Clinic (United States)

Part A Optical Techniques in Neurosurgery, Brain Imaging, and Neurobiology

  • Conference Chairs

    • Henry Hirschberg, Beckman Laser Institute and Medical Clinic (United States)

    • Steen J. Madsen, University of Nevada, Las Vegas (United States)

  • Conference Program Committee

    • David Abookasis, Ariel University of Samaria (Israel)

    • Frederic Leblond, Ecole Polytechnique de Montréal (Canada)

    • Herbert Stepp, Ludwig-Maximilians-Universität München (Germany)

    • Pablo A. Valdes, Dartmouth College (United States)

    • Victor X. D. Yang, Ryerson University (Canada)

  • Session Chairs

    • 1 OCT and Microscopy I

      • Victor X. D. Yang, Ryerson University (Canada)

    • 2 OCT and Microscopy II

      • Steen J. Madsen, University of Nevada, Las Vegas (United States)

    • 3 Operative and Post-op Therapy I

      • Henry Hirschberg, Beckman Laser Institute and Medical Clinic (United States)

    • 4 Operative and Post-Op Therapy II

      • Frédéric Leblond, Ecole Polytechnique de Montréal (Canada)

    • 5 Optical Spectroscopy and Tomography

      • David Abookasis, Ariel University (Israel)

    • 6 OCT and Microscopy III

      • Pablo A. Valdes, Dartmouth College (United States)

    • 7 Operative and Post-Op Therapy III

      • Pablo A. Valdes, Dartmouth College (United States)

Part B Neurophotonics

  • Conference Chairs

    • E. Duco Jansen, Vanderbilt University (United States)

    • Qingming Luo, Huazhong University of Science and Technology (China)

  • Conference Co-chairs

    • Jun Ding, Stanford School of Medicine (United States)

    • Anna W. Roe, Vanderbilt University (United States)

  • Conference Program Committee

    • David A. Boas, Massachusetts General Hospital (United States)

    • Yu Chen, University of Maryland, College Park (United States)

    • Javier DeFelipe, University Politécnica de Madrid (Spain)

    • Hongwei Dong, University of California, Los Angeles (United States)

    • Congwu Du, Stony Brook University (United States)

    • Beop-Min Kim, Korea University College of Health Sciences (Korea, Republic of)

    • Vesa Kiviniemi, University of Oulu (Finland)

    • Pengcheng Li, Britton Chance Center for Biomedical Photonics (China)

    • Anita Mahadevan-Jansen, Vanderbilt University (United States)

    • Francesco Saverio Pavone, Laboratorio Europeo per la Spettroscopia Non-lineare (Italy)

    • Kambiz Pourrezaei, Drexel University (United States)

    • Claus-Peter Richter, Northwestern University (United States)

    • Shy Shoham, Technion-Israel Institute of Technology (Israel)

    • Vladislav Toronov, Ryerson University (Canada)

    • Shaoqun Zeng, Britton Chance Center for Biomedical Photonics (China)

  • Session Chairs

    • 1 Novel Photonic or Optoelectronic Methods

      • E. Duco Jansen, Vanderbilt University (United States)

    • 2 Neurophotonic Applications I

      • Francesco Saverio Pavone, Laboratorio Europeo per la Spettroscopia Non-lineare (Italy)

    • 3 Neurophotonic Applications II

      • Beop-Min Kim, Korea University College of Health Sciences (Korea, Republic of)

    • 4 Diffusive Optical Tomography and Functional Near-Infrared Imaging

      • Hope T. Beier, Air Force Research Laboratory (United States)

    • 5 High Resolution and Multimodal Imaging

      • Shy Shoham, Technion-Israel Institute of Technology (Israel)

    • 6 Novel Optical Methods for Studying Cortical Function

      • Anna Wang Roe, Vanderbilt University (United States)

    • 7 Neurophotonics Plenary Speaker

      • Qingming Luo, Britton Chance Center for Biomedical Photonics (China)

    • 8 Visible Brain-Wide Networks

      • Yu Chen, University of Maryland, College Park (United States)

Part C Optogenetics and Optical Control of Cells

  • Conference Chairs

    • Samarendra K. Mohanty, The University of Texas at Arlington (United States)

    • Nitish V. Thakor, Johns Hopkins University (United States)

  • Conference Program Committee

    • Antoine Adamantidis, McGill University (Canada)

    • George J. Augustine, Duke-NUS Graduate Medical School (Singapore)

    • Klaus B. Gerwert, Ruhr-Universität Bochum (Germany)

    • Xue Han, Boston University (United States)

    • Elizabeth M. Hillman, Columbia University (United States)

    • Richard Kramer, University of California, Berkeley (United States)

    • Alfred L. Nuttall, Oregon Health & Science University (United States)

    • Anna W. Roe, Vanderbilt University (United States)

    • Ulrich T. Schwarz, Fraunhofer IAF (Germany) and IMTEK, Albert- Ludwigs-Universität Freiburg (Germany)

    • John P. Welsh, University of Washington (United States)

    • Rafael Yuste M.D., Columbia University (United States)

  • Session Chairs

    • 1 Optogenetics I

      • Nitish V. Thakor, Johns Hopkins University (United States)

      • Samarendra K. Mohanty, The University of Texas at Arlington (United States)

    • 2 Optogenetics II

      • Nitish V. Thakor, Johns Hopkins University (United States)

      • Samarendra K. Mohanty, The University of Texas at Arlington (United States)

    • 3 Optogenetics III

      • George J. Augustine, Lee Kong Chian Medical School (Singapore)

    • 4 Optogenetics IV

      • George J. Augustine, Lee Kong Chian Medical School (Singapore)


While light has been used for several decades for non-contact stimulation and manipulation of cells, optogenetics adds to the innovative optical toolkit for optical control of cells. Optogenetics refers to the use of optics and genetics together for controlling activity of proteins and cellular function. Optogenetics present cell-specific optical control of the functioning of genetically-targeted/modified cells. The use of optogenetics has exploded in last few years and has given rise to great advances in basic and applied research. The cellular functions that can be controlled with optogenetics include stimulation/inhibition of cells, gene activation, intracellular signaling, and migration. Many different cells including neurons, cardiac cells, stem cells and cancer cells can be controlled by optogenetic modulation. Optogenetics technologies could eventually form the basis for vision restoration, psychiatric treatment and pain-control. Photonic technologies are playing crucial role in both delivering light for cellular control, and, in some cases, for imaging the consequences of this control. The conference was divided into four major sessions spread over two days.

Innovative schemes for delivery and control of light irradiation, including miniaturized light sources, fiber optics, waveguides and special beams can potentially transform optogenetic approaches. Prof. John Rogers (UIUC) described the advancement on implantable, wireless optoelectronic systems for optogenetics, as part of the opening keynote lecture. Results of chronic optogenetic stimulation and recording across cortical areas in non-human primates using high-density micro-electrocorticography were presented in this session. Further, ultra-compact multi-channel implantable optical neural probes for deep brain optogenetic stimulation using visible array waveguide gratings or multipoint light emitting optical fibers were described in several talks. Prof. Patrick Ruther (Univ. of Freiburg) presented an invited talk on "Highly compact MEMS-based optrodes with integrated light sources" in which he reviewed the state-of-the-art technologies for optrodes enabling simultaneous illumination and electrical recording from brain. Together, these talks depicted the role of nanophotonics and electronics in successful implementation of optogenetic technologies.

Prof. Edward S. Boyden (MIT) gave the second Keynote lecture on "Optical tools for mapping and engineering the brain". His talk described his recent work on high-resolution imaging of brain using expansion microscopy. Further, he presented advancement in the development of new opsins in his lab providing red-shifted activation. With development of near-infrared light controllable proteins, cellular systems can be modulated in a minimally-invasive manner. Yawo group presented use of up-conversion of near-infrared light by lanthanide nanoparticles to activate channelrhodopsins for manipulating neural activities. Researchers from Mohanty Laboratory presented development of multi-opsin and use of broad-band light for ambient white-light based restoration of vision in photodegenerative retinal diseases. While molecular biology researchers will continue to engineer more efficient opsins having unique temporal, functional, and spectral characteristics, there is also a need to develop new molecular probes and imaging method for mapping cellular activation. Label-free optical polarimetric detection of cellular activation by optogenetic excitation was presented in this conference. A representative from industry (AXXAM, Italy) presented the use of different light-gated actuators and sensors in the generation of novel cellular recombinant assays, in order to prove their robustness and adaptability for drug discovery assays.

Several studies on in-vivo optical stimulation and optical/electrical read-out from neural circuits in non-human primates and rodents were presented. In a keynote lecture titled "Playing the piano with neural circuits: simultaneous 3D all-optical imaging and activation of neurons in vivo", Prof. Yuste (Columbia University) reviewed the efforts of his group in developing optical methods to perform two-photon imaging and photostimulation of neuronal populations using spatial light modulators, PSF engineering and a variety of optical, optogenetic and optochemical sensors. He also delivered a talk on "Simultaneous Imaging of Neural Activity in 3D” as part of BiOS hot topics. Detection and modulation of spatiotemporal patterns of neuronal activity in primary visual cortex from awake behaving mice was presented. Dr. Nassi (Salk Institute) presented measurement on the causal effects of locally-generated excitation and inhibition on spontaneous and visually-evoked responses in visual cortex of alert, fixating macaque monkeys. Prof. Augustine (KIST and Duke) presented an invited talk on optogenetics to map the spatial organization of local circuits.

Innovations in optical techniques hold significant promise for advancing optogenetic technologies. The introduction of non-linear optics has allowed precise and in-depth spatial control of optogenetic stimulation with (sub)cellular resolution. In vivo all-optical interrogation of neurons in mice using two-photon optogenetic stimulation and calcium imaging with two-photon fluorescence microscopy was presented by researchers from Canada. Prof. Shoham (Israel Institute of Technology) presented an invited talk on "Distributed optogenetic interfacing with retinas, optonets and brains: photonics and potential applications". Researchers from UIUC described the use of a photonic crystal fiber pumped by Ytterbium laser as a broadband femtosecond source for two-photon optogenetics and imaging. Researchers from Europe presented Spatio-angular light control in microscopes using micro mirror arrays for precise, localized activation of optogenetic probes or the activation and deactivation of signaling cascades using photo-activated ion-channels. An automated laser tracking and photothermal/optogenetic manipulation system for studying social behaviors in multiple freely moving fruit flies was described in an invited talk by Dr. Lin (National Tsing Hua Univ). Researchers from Mohanty Laboratory described use of ultrafast NIR laser microbeam for delivery of opsin-encoding genes and impermeable actin-staining molecules into spatially-targeted neurons enabling visualization of neuronal network and their activation.

Besides control of light-activatable ion-channels, optogenetics encompasses controlling activity of other proteins and cellular functions. The varieties of cellular functions that can be controlled with optogenetics include stimulation/inhibition of cells, gene activation, intracellular signaling, and migration. Prof. Hahn (UNC at Chapel Hill) presented a keynote lecture on "Engineering proteins for visualization and control of signaling networks in vivo". He described new tools to visualize and manipulate signaling in cells, using Rho family GTPase networks and cell motility as test beds. Further, methods to direct activated kinases to specific targets and control sequestration of proteins at intracellular membranes with light was described. Prof. Lin (Stanford) described optogenetic control of protein activity with photodissociable fluorescent proteins. Such fluorescent light-inducible proteins enabled optical control over guanine nucleotide exchange factor and protease domains, and can be generalized in future to other protein families including kinase and DNA nuclease. Studies on optomechanical and photothermal manipulation of cells were also presented by various researchers. Scientists from Japan presented work on "Laser-induced perturbation into molecular dynamics localized in neuronal cell" using optical trapping dynamics of synaptic vesicles or neural cell adhesion molecules. Further, dielectrophoretic trapping using metal coated chemically etched fiber was proposed for cell manipulation and isolation. At the end of the conference, Prof. Mohanty presented all-optical approach for construction (by optical tweezers, and axonal guidance), and manipulation (laser nanosurgery and optoporation) as well as modulation (optical or optogenetic) and detection (calcium and quantitative phase imaging) of neural activities.

While looking forward, we believe applications of optogenetic and other optical modulation of cells will have wide-variety of basic research and biomedical applications. Though representatives from industry also participated in the conference, more active role of the photonics industry in developing novel source and imaging platforms for optical activation and detection of cellular activities will be crucial for the successful development of this area. We hope that reading the talks presented in the annual Optogenetics and Optical control of cells conference and abstracts/articles provided in this volume will convey the knowledge and excitement of this exciting field. We look forward to your participation as authors and presenters in this conference next year. In addition to the travel support provided by SPIE to selected students, we were able to partially support selected speakers. We believe that we will be able to support more participants next year with support from the sponsors.


We would like to thank BiOS symposium chairs Prof. Rox Anderson (Harvard) and Prof. Jim Fujimoto (MIT) for continuous encouragement to organize the conference on Optogenetics and Optical control of cells as part of the BiOS symposium during Photonics West 2015. We would also like to thank Prof. George Augustine (KIST and Duke University), Prof. Rafael Yuste (Columbia University), Prof. Ulrich T. Schwarz (Univ. of Freiburg), Prof. Anna Roe (Vanderbilt University), Prof. John Welsh (University of Washington-Seattle), Prof. Richard Kramer (UC Berkeley), Prof. Xue Han (Boston University), Prof. Klaus Gerwert (Ruhr-Univ. Bochum), Prof. Elizabeth Hillman (Columbia University), Prof. Alfred L. Nuttall (Oregon Health & Science Univ), Prof. Antoine Adamantidis (McGill University) for their valuable input as members of the Program Committee. We would like to thank all keynote and invited speakers, session chairs, and participants for their important contributions. We would like to thank our sponsors, SPIE and Plexon Inc. (Mr. Harvey Wiggins) for their support.

Samarendra K. Mohanty

Nitish V. Thakor

© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
} "Front Matter: Volume 9305", Proc. SPIE 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II, 930501 (9 April 2015); doi: 10.1117/12.2183897; https://doi.org/10.1117/12.2183897

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