Low level light promotes the proliferation and differentiation of bone marrow derived mesenchymal stem cells

Jin-Chul Ahn; Yun-Hee Rhee; Sun-Hyang Choi; Dae Yu Kim; Phil-Sang Chung M.D.

doi:10.1117/12.2078863

5 March 2015 Low level light promotes the proliferation and differentiation of bone marrow derived mesenchymal stem cells

Jin-Chul Ahn, Yun-Hee Rhee, Sun-Hyang Choi, Dae Yu Kim, Phil-Sang Chung M.D.

Author Affiliations +

Proceedings Volume 9309, Mechanisms for Low-Light Therapy X; 93090V (2015) https://doi.org/10.1117/12.2078863
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Low-level light irradiation (LLLI) reported to stimulate the proliferation or differentiation of a variety of cell types. However, very little is known about the effect of light therapy on stem cells. The aim of the present study was to evaluate the effect of LLLI on the molecular physiological change of human bone marrow derived stem cells (hBMSC) by wavelength (470, 630, 660, 740 and 850, 50mW). The laser diode was performed with different time interval (0, 7.5, 15, 30J/cm², 50mW) on hBMSC. To determine the molecular physiological changes of cellular level of hBMSC, the clonogenic assay, ATP assay, reactive oxygen species (ROS) detection, mitochondria membrane potential (MMPΦ) staining and calcium efflux assay were assessed after irradiation. There was a difference between with and without irradiation on hBMSCs. An energy density up to 30 J/cm² improved the cell proliferation in comparison to the control group. Among these irradiated group, 630 and 660nm were significantly increased the cell proliferation. The cellular level of ATP and calcium influx was increased with energy dose-dependent in all LLLI groups. Meanwhile, ROS and MMPΦ were also increased after irradiation except 470nm. It can be concluded that LLLI using infrared light and an energy density up to 30 J/cm² has a positive stimulatory effect on the proliferation or differentiation of hBMSCs. Our results suggest that LLLI may influence to the mitochondrial membrane potential activity through ATP synthesis and increased cell metabolism which leads to cell proliferation and differentiation.

Citation Download Citation

Jin-Chul Ahn, Yun-Hee Rhee, Sun-Hyang Choi, Dae Yu Kim, and Phil-Sang Chung M.D. "Low level light promotes the proliferation and differentiation of bone marrow derived mesenchymal stem cells", Proc. SPIE 9309, Mechanisms for Low-Light Therapy X, 93090V (5 March 2015); https://doi.org/10.1117/12.2078863

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