Effectiveness of antimicrobial  photodynamic therapy on staphylococcus aureus using phenothiazinium dye with red laser

Juliana S. C. Monteiro; Susana C. P. S. de Oliveira D.D.S.; Gustavo M. Pires-Santos; Fernando José Pires Sampaio D.D.S.; Fátima Antônia Aparecida Zanin; Antônio L. B. Pinheiro D.D.S.

doi:10.1117/12.2079170

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5 March 2015 Effectiveness of antimicrobial photodynamic therapy on staphylococcus aureus using phenothiazinium dye with red laser

Juliana S. C. Monteiro, Susana C. P. S. de Oliveira D.D.S., Gustavo M. Pires-Santos, Fernando José Pires Sampaio D.D.S., Fátima Antônia Aparecida Zanin, Antônio L. B. Pinheiro D.D.S.

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Proceedings Volume 9309, Mechanisms for Low-Light Therapy X; 93090X (2015) https://doi.org/10.1117/12.2079170
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The aim of this study was to evaluate in vitro the bactericidal effect of Antimicrobial Photodynamic Therapy - AmPDT using a phenothiazinium compound (toluidine blue O and methylene blue, 12.5 μg/mL) on Staphylococcus aureus (ATCC 23529) irradiated or not with the red laser (λ 660 nm, 12J/cm²). All tests were performed in triplicate and samples distributed into the following groups: Negative control, Laser, Photosensitizer, and AmPDT. Bactericidal effect of the Antimicrobial Photodynamic Therapy was assessed by counting of colony-forming units and analyzed statistically (ANOVA, Tukey test, p<0.05). The results showed, comparing the Laser group with Negative control, a statistically significant increase of counting on the Laser group (p = 0.003). The use of the photosensitizer alone reduced the mean number of CFU (64.8%) and its association with the Laser light resulted in 84.2% of inhibition. The results are indicative that the use of Antimicrobial Photodynamic Therapy presented in vitro bactericidal effect on Staphylococcus aureus.

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Juliana S. C. Monteiro, Susana C. P. S. de Oliveira D.D.S., Gustavo M. Pires-Santos, Fernando José Pires Sampaio D.D.S., Fátima Antônia Aparecida Zanin, and Antônio L. B. Pinheiro D.D.S. "Effectiveness of antimicrobial photodynamic therapy on staphylococcus aureus using phenothiazinium dye with red laser", Proc. SPIE 9309, Mechanisms for Low-Light Therapy X, 93090X (5 March 2015); https://doi.org/10.1117/12.2079170

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