2 March 2015 Examining small molecule: HIV RNA interactions using arrayed imaging reflectometry
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Abstract
Human Immunodeficiency Virus (HIV) has been the subject of intense research for more than three decades as it causes an uncurable disease: Acquired Immunodeficiency Syndrome, AIDS. In the pursuit of a medical treatment, RNAtargeted small molecules are emerging as promising targets. In order to understand the binding kinetics of small molecules and HIV RNA, association (ka) and dissociation (kd) kinetic constants must be obtained, ideally for a large number of sequences to assess selectivity. We have developed Aqueous Array Imaged Reflectometry (Aq-AIR) to address this challenge. Using a simple light interference phenomenon, Aq-AIR provides real-time high-throughput multiplex capabilities to detect binding of targets to surface-immobilized probes in a label-free microarray format. The second generation of Aq-AIR consisting of high-sensitivity CCD camera and 12-μL flow cell was fabricated. The system performance was assessed by real-time detection of MBNL1-(CUG)10 and neomycin B - HIV RNA bindings. The results establish this second-generation Aq-AIR to be able to examine small molecules binding to RNA sequences specific to HIV.
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Wanaruk Chaimayo, Wanaruk Chaimayo, Benjamin L. Miller, Benjamin L. Miller, } "Examining small molecule: HIV RNA interactions using arrayed imaging reflectometry", Proc. SPIE 9310, Frontiers in Biological Detection: From Nanosensors to Systems VII, 931006 (2 March 2015); doi: 10.1117/12.2085058; https://doi.org/10.1117/12.2085058
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