4 March 2015 Radiometric calibration to consider in quantitative clinical fluorescence imaging measurements
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Abstract
The fluorescent light detected by a clinical imager is assumed to be proportional only to the amount of fluorescent substance present in the sample and the level of excitation. Unfortunately, there are many factors that can add or subtract to the light signal directly attributable to the desired fluorescence emission, especially with fluorescence from inside the body imaged remotely. The quantification of fluorescence emission is feasible by calibrating the imager using international system of units (SI)-traceable physical and material calibration artifacts such that the detector’s digital numbers (DN) can be converted to radiometric units. Here we discuss three calibration methods for quantitative clinical fluorescence imaging systems.
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M. Litorja, A. Urbas, Y. Zong, "Radiometric calibration to consider in quantitative clinical fluorescence imaging measurements", Proc. SPIE 9311, Molecular-Guided Surgery: Molecules, Devices, and Applications, 931114 (4 March 2015); doi: 10.1117/12.2086838; https://doi.org/10.1117/12.2086838
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