2 March 2015 Ultrasensitive quantification of cerebral capillary flow networks and dynamics
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Ultra-high resolution optical Doppler coherence tomography (μODT) is a promising tool for brain functional imaging. However, its sensitivity for detecting slow flows in capillary beds may limit its utility in visualizing and quantifying subtle changes in brain microcirculation. To address this limitation, we developed a novel method called contrast-enhanced μODT (c-μODT) in which intralipid is injected into mouse tail vein to enhance μODT detection sensitivity. We demonstrate that after intralipid injection, the flow detection sensitivity of μODT is dramatically enhanced by 230% as quantified by the fill factor (FF) of microvasculature. More importantly, we show that c-μODT preserves the quantitative properties for flow imaging, i.e., showing a comparable change ratio of hypercapnia-induced flow increase in the capillary network before and after injecting intralipid.
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Jiang You, Jiang You, Ki Park, Ki Park, Congwu Du, Congwu Du, Yingtian Pan, Yingtian Pan, "Ultrasensitive quantification of cerebral capillary flow networks and dynamics", Proc. SPIE 9312, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX, 931218 (2 March 2015); doi: 10.1117/12.2082428; https://doi.org/10.1117/12.2082428

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