Standardizing flow cytometric assays in long-term population-based studies

Susanne Melzer; Jozsef Bocsi; Attila Tárnok

doi:10.1117/12.2075426

Translator Disclaimer

5 March 2015 Standardizing flow cytometric assays in long-term population-based studies

Susanne Melzer, Jozsef Bocsi, Attila Tárnok

Author Affiliations +

Proceedings Volume 9315, Design and Quality for Biomedical Technologies VIII; 93150H (2015) https://doi.org/10.1117/12.2075426
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Quantification of leukocyte subpopulations and characterization of antigen-expression pattern on the cellular surface can play an important role in diagnostics. The state of cellular immunology on the single-cell level was analyzed by polychromatic flow cytometry in a recent comparative study within the average Leipzig population (LIFE - Leipzig Research Centre for Civilization Diseases). Data of 1699 subjects were recorded over a long-time period of three years (in a total of 1126 days). To ensure compatibility of such huge data sets, quality-controls on many levels (stability of instrumentation, low intra-laboratory variance and reader independent data analysis) are essential. The LIFE study aims to analyze various cytometric pattern to reveal the relationship between the life-style, the environmental effects and the individual health. We therefore present here a multi-step quality control procedure for long-term comparative studies.

Citation Download Citation

Susanne Melzer, Jozsef Bocsi, and Attila Tárnok "Standardizing flow cytometric assays in long-term population-based studies", Proc. SPIE 9315, Design and Quality for Biomedical Technologies VIII, 93150H (5 March 2015); https://doi.org/10.1117/12.2075426

ACCESS THE FULL ARTICLE