Development of a multi-scale and multi-modality imaging system to characterize tumours and their microenvironment in vivo

Valérie Rouffiac; Karine Ser-Leroux; Emilie Dugon; Ingrid Leguerney; Mélanie Polrot; Sandra Robin; Sophie Salomé-Desnoulez; Jean-Christophe Ginefri; Catherine Sebrié; Corinne Laplace-Builhé

doi:10.1117/12.2077653

5 March 2015 Development of a multi-scale and multi-modality imaging system to characterize tumours and their microenvironment in vivo

Valérie Rouffiac, Karine Ser-Leroux, Emilie Dugon, Ingrid Leguerney, Mélanie Polrot, Sandra Robin, Sophie Salomé-Desnoulez, Jean-Christophe Ginefri, Catherine Sebrié, Corinne Laplace-Builhé

Author Affiliations +

Proceedings Volume 9316, Multimodal Biomedical Imaging X; 93160I (2015) https://doi.org/10.1117/12.2077653
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

In vivo high-resolution imaging of tumor development is possible through dorsal skinfold chamber implantable on mice model. However, current intravital imaging systems are weakly tolerated along time by mice and do not allow multimodality imaging. Our project aims to develop a new chamber for: 1- long-term micro/macroscopic visualization of tumor (vascular and cellular compartments) and tissue microenvironment; and 2- multimodality imaging (photonic, MRI and sonography). Our new experimental device was patented in March 2014 and was primarily assessed on 75 mouse engrafted with 4T1-Luc tumor cell line, and validated in confocal and multiphoton imaging after staining the mice vasculature using Dextran 155KDa-TRITC or Dextran 2000kDa-FITC. Simultaneously, a universal stage was designed for optimal removal of respiratory and cardiac artifacts during microscopy assays. Experimental results from optical, ultrasound (Bmode and pulse subtraction mode) and MRI imaging (anatomic sequences) showed that our patented design, unlike commercial devices, improves longitudinal monitoring over several weeks (35 days on average against 12 for the commercial chamber) and allows for a better characterization of the early and late tissue alterations due to tumour development. We also demonstrated the compatibility for multimodality imaging and the increase of mice survival was by a factor of 2.9, with our new skinfold chamber. Current developments include: 1- defining new procedures for multi-labelling of cells and tissue (screening of fluorescent molecules and imaging protocols); 2- developing ultrasound and MRI imaging procedures with specific probes; 3- correlating optical/ultrasound/MRI data for a complete mapping of tumour development and microenvironment.

Citation Download Citation

Valérie Rouffiac, Karine Ser-Leroux, Emilie Dugon, Ingrid Leguerney, Mélanie Polrot, Sandra Robin, Sophie Salomé-Desnoulez, Jean-Christophe Ginefri, Catherine Sebrié, and Corinne Laplace-Builhé "Development of a multi-scale and multi-modality imaging system to characterize tumours and their microenvironment in vivo", Proc. SPIE 9316, Multimodal Biomedical Imaging X, 93160I (5 March 2015); https://doi.org/10.1117/12.2077653

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Magnetic resonance imaging

Tumors

Imaging systems

In vivo imaging

Tissue optics

Confocal microscopy

Ultrasonography

Show All Keywords

Keywords/Phrases

Search In:

Publication Years