Correlative super-resolution fluorescence microscopy combined with optical coherence microscopy

Sungho Kim; Gyeongtae Kim; Soohyun Jang; Sang-Hee Shim; Sung Chul Bae

doi:10.1117/12.2079340

11 March 2015 Correlative super-resolution fluorescence microscopy combined with optical coherence microscopy

Sungho Kim, Gyeongtae Kim, Soohyun Jang, Sang-Hee Shim, Sung Chul Bae

Proceedings Volume 9316, Multimodal Biomedical Imaging X; 93160R (2015) https://doi.org/10.1117/12.2079340
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Recent development of super-resolution fluorescence imaging technique such as stochastic optical reconstruction microscopy (STORM) and photoactived localization microscope (PALM) has brought us beyond the diffraction limits. It allows numerous opportunities in biology because vast amount of formerly obscured molecular structures, due to lack of spatial resolution, now can be directly observed. A drawback of fluorescence imaging, however, is that it lacks complete structural information. For this reason, we have developed a super-resolution multimodal imaging system based on STORM and full-field optical coherence microscopy (FF-OCM). FF-OCM is a type of interferometry systems based on a broadband light source and a bulk Michelson interferometer, which provides label-free and non-invasive visualization of biological samples. The integration between the two systems is simple because both systems use a wide-field illumination scheme and a conventional microscope. This combined imaging system gives us both functional information at a molecular level (~20nm) and structural information at the sub-cellular level (~1μm). For thick samples such as tissue slices, while FF-OCM is readily capable of imaging the 3D architecture, STORM suffer from aberrations and high background fluorescence that substantially degrade the resolution. In order to correct the aberrations in thick tissues, we employed an adaptive optics system in the detection path of the STORM microscope. We used our multimodal system to obtain images on brain tissue samples with structural and functional information.

Citation Download Citation

Sungho Kim, Gyeongtae Kim, Soohyun Jang, Sang-Hee Shim, and Sung Chul Bae "Correlative super-resolution fluorescence microscopy combined with optical coherence microscopy", Proc. SPIE 9316, Multimodal Biomedical Imaging X, 93160R (11 March 2015); https://doi.org/10.1117/12.2079340

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