5 March 2015 Tissue classification and diagnostics using a fiber probe for combined Raman and fluorescence spectroscopy
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Abstract
Two different optical fiber probes for combined Raman and fluorescence spectroscopic measurements were designed, developed and used for tissue diagnostics. Two visible laser diodes were used for fluorescence spectroscopy, whereas a laser diode emitting in the NIR was used for Raman spectroscopy. The two probes were based on fiber bundles with a central multimode optical fiber, used for delivering light to the tissue, and 24 surrounding optical fibers for signal collection. Both fluorescence and Raman spectra were acquired using the same detection unit, based on a cooled CCD camera, connected to a spectrograph. The two probes were successfully employed for diagnostic purposes on various tissues in a good agreement with common routine histology. This study included skin, brain and bladder tissues and in particular the classification of: malignant melanoma against melanocytic lesions and healthy skin; urothelial carcinoma against healthy bladder mucosa; brain tumor against dysplastic brain tissue. The diagnostic capabilities were determined using a cross-validation method with a leave-one-out approach, finding very high sensitivity and specificity for all the examined tissues. The obtained results demonstrated that the multimodal approach is crucial for improving diagnostic capabilities. The system presented here can improve diagnostic capabilities on a broad range of tissues and has the potential of being used for endoscopic inspections in the near future.
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Riccardo Cicchi, Suresh Anand, Susanna Rossari, Alessandro Sturiale, Flavio Giordano, Vincenzo De Giorgi, Vincenza Maio, Daniela Massi, Gabriella Nesi, Anna Maria Buccoliero, Francesco Tonelli, Renzo Guerrini, Nicola Pimpinelli, and Francesco Saverio Pavone "Tissue classification and diagnostics using a fiber probe for combined Raman and fluorescence spectroscopy", Proc. SPIE 9318, Optical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 931808 (5 March 2015); doi: 10.1117/12.2079009; https://doi.org/10.1117/12.2079009
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