Fluorescence lifetime spectroscopy for breast cancer margins assessment

Dimitris Gorpas; Hussain Fatakdawala; Yanhong Zhang; Richard Bold; Laura Marcu

doi:10.1117/12.2079622

12 March 2015 Fluorescence lifetime spectroscopy for breast cancer margins assessment

Dimitris Gorpas, Hussain Fatakdawala, Yanhong Zhang, Richard Bold, Laura Marcu

Proceedings Volume 9318, Optical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis; 93180B (2015) https://doi.org/10.1117/12.2079622
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

During breast conserving surgery (BCS), which is the preferred approach to treat most early stage breast cancers, the surgeon attempts to excise the tumor volume, surrounded by thin margin of normal tissue. The intra-operative assessment of cancerous areas is a challenging procedure, with the surgeon usually relying on visual or tactile guidance. This study evaluates whether time-resolved fluorescence spectroscopy (TRFS) presents the potential to address this problem. Point TRFS measurements were obtained from 19 fresh tissue slices (7 patients) and parameters that characterize the transient signals were quantified via constrained least squares deconvolution scheme. Fibrotic tissue (FT, n=69), adipose tissue (AT, n=76), and invasive ductal carcinoma (IDC, n=27) were identified in histology and univariate statistical analysis, followed by multi-comparison test, was applied to the corresponding lifetime data. Significant differentiation between the three tissue types exists at 390 nm and 500 nm bands. The average lifetime is 3.23±0.74 ns for AT, 4.21±0.83 ns for FT and 4.71±0.35 ns (p<0.05) for IDC at 390 nm. Due to the smaller contribution of collagen in AT the average lifetime value is different from FT and IDC. Additionally, although intensity measurements do not show difference between FT and IDC, lifetime can distinguish them. Similarly, in 500 nm these values are 7.01±1.08 ns, 5.43±1.05 ns and 4.39±0.88 ns correspondingly (p<0.05) and this contrast is due to differentiation in retinol or flavins relative concentration, mostly contributing to AT. Results demonstrate the potential of TRFS to intra-operatively characterize BCS breast excised tissue in real-time and assess tumor margins.

Citation Download Citation

Dimitris Gorpas, Hussain Fatakdawala, Yanhong Zhang, Richard Bold, and Laura Marcu "Fluorescence lifetime spectroscopy for breast cancer margins assessment", Proc. SPIE 9318, Optical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 93180B (12 March 2015); https://doi.org/10.1117/12.2079622

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available