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12 March 2015 Excitation light leakage suppression using temperature sensitive fluorescent agents
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Abstract
Fluorescence tomography is a non invasive, non ionizing imaging technique able to provide a 3D distribution of fluorescent agents within thick highly scattering mediums, using low cost instrumentation. However, its low spatial resolution due to undetermined and ill-posed nature of its inverse problem has delayed its integration into the clinical settings. In addition, the quality of the fluorescence tomography images is degraded due to the excitation light leakage contaminating the fluorescence measurements. This excitation light leakage results from the excitation photons that cannot be blocked by the fluorescence filters. In this contribution, we present a new method to remove this excitation light leakage noise based on the use of a temperature sensitive fluorescence agents. By performing different sets of measurements using this temperature sensitive agents at multiple temperatures, the excitation light leakage can be estimated and then removed from the measured fluorescence signals . The results obtained using this technique demonstrate its potential for use in in-vivo small animal imaging.
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Farouk Nouizi, Tiffany C. Kwong, Jessica Kwong, Jaedu Cho, Yuwen Chan, Uma Sampathkumaran, Yue Zhu, Maksudul M. Alam, and Gultekin Gulsen "Excitation light leakage suppression using temperature sensitive fluorescent agents", Proc. SPIE 9319, Optical Tomography and Spectroscopy of Tissue XI, 93190Y (12 March 2015); doi: 10.1117/12.2080630; https://doi.org/10.1117/12.2080630
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