5 March 2015 Evidence of thermal additivity during short laser pulses in an in vitro retinal model
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Laser damage thresholds were determined for exposure to 2.5-ms 532-nm pulses in an established in vitro retinal model. Single and multiple pulses (10, 100, 1000) were delivered to the cultured cells at three different pulse repetition frequency (PRF) values, and overt damage (membrane breach) was scored 1 hr post laser exposure. Trends in the damage data within and across the PRF range identified significant thermal additivity as PRF was increased, as evidenced by drastically reduced threshold values (< 40% of single-pulse value). Microthermography data that were collected in real time during each exposure also provided evidence of thermal additivity between successive laser pulses. Using thermal profiles simulated at high temporal resolution, damage threshold values were predicted by an in-house computational model. Our simulated ED50 value for a single 2.5-ms pulse was in very good agreement with experimental results, but ED50 predictions for multiple-pulse trains will require more refinement.
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Michael L. Denton, Amanda J. Tijerina, Phillip N. Dyer, Chad A. Oian, Gary D. Noojin, John M. Rickman, Aurora D. Shingledecker, Clifton D. Clark, Cherry C. Castellanos, Robert J. Thomas, and Benjamin A. Rockwell "Evidence of thermal additivity during short laser pulses in an in vitro retinal model", Proc. SPIE 9321, Optical Interactions with Tissue and Cells XXVI, 93210G (5 March 2015); doi: 10.1117/12.2081243; https://doi.org/10.1117/12.2081243

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