11 March 2015 Photoacoustic imaging of single circulating melanoma cells in vivo
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Abstract
Melanoma, one of the most common types of skin cancer, has a high mortality rate, mainly due to a high propensity for tumor metastasis. The presence of circulating tumor cells (CTCs) is a potential predictor for metastasis. Label-free imaging of single circulating melanoma cells in vivo provides rich information on tumor progress. Here we present photoacoustic microscopy of single melanoma cells in living animals. We used a fast-scanning optical-resolution photoacoustic microscope to image the microvasculature in mouse ears. The imaging system has sub-cellular spatial resolution and works in reflection mode. A fast-scanning mirror allows the system to acquire fast volumetric images over a large field of view. A 500-kHz pulsed laser was used to image blood and CTCs. Single circulating melanoma cells were imaged in both capillaries and trunk vessels in living animals. These high-resolution images may be used in early detection of CTCs with potentially high sensitivity. In addition, this technique enables in vivo study of tumor cell extravasation from a primary tumor, which addresses an urgent pre-clinical need.
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Lidai Wang, Lidai Wang, Junjie Yao, Junjie Yao, Ruiying Zhang, Ruiying Zhang, Song Xu, Song Xu, Guo Li, Guo Li, Jun Zou, Jun Zou, Lihong V. Wang, Lihong V. Wang, } "Photoacoustic imaging of single circulating melanoma cells in vivo", Proc. SPIE 9323, Photons Plus Ultrasound: Imaging and Sensing 2015, 93230A (11 March 2015); doi: 10.1117/12.2076987; https://doi.org/10.1117/12.2076987
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