11 March 2015 Noninvasive photoacoustic microscopy of methemoglobin in vivo
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Various causes can lead to methemoglobinemia, and it has the potential to be confused with other diseases. In vivo measurements of methemoglobin have significant applications in the clinics. We quantified the average and the distributed percentage of methemoglobin both in vitro and in vivo using photoacoustic microscopy (PAM). Based on the absorption spectra of methemoglobin, oxyhemoglobin, and deoxyhemoglobin, three wavelengths were chosen to differentiate methemoglobin from the others. We imaged the methemoglobin percentage in microtubes that mimicked blood vessels as a phantom experiment. The methemoglobin concentrations calculated from the photoacoustic signals were in accordance with the preset concentrations. We also demonstrated the ability of PAM to quantitatively image methemoglobin distribution in vivo in a mouse ear.
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Min Tang, Min Tang, Yong Zhou, Yong Zhou, Ruiying Zhang, Ruiying Zhang, Lihong V. Wang, Lihong V. Wang, "Noninvasive photoacoustic microscopy of methemoglobin in vivo", Proc. SPIE 9323, Photons Plus Ultrasound: Imaging and Sensing 2015, 93233K (11 March 2015); doi: 10.1117/12.2076692; https://doi.org/10.1117/12.2076692

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