Intravital imaging of multicolor-labeled tumor immune microenvironment through skin-fold window chamber

Shuhong Qi; Zhihong Zhang

doi:10.1117/12.2078084

9 March 2015 Intravital imaging of multicolor-labeled tumor immune microenvironment through skin-fold window chamber

Shuhong Qi, Zhihong Zhang

Proceedings Volume 9324, Biophotonics and Immune Responses X; 93240F (2015) https://doi.org/10.1117/12.2078084
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Tumor immune microenvironment became very important for the tumor immunotherapy. There were several kinds of immune cells in tumor stromal, and they played very different roles in tumor growth. In order to observe the behaviors of multiple immune cells in tumor microenvironment and the interaction between immune cells and tumor cells at the same time, we generated a multicolor-labeled tumor immune microenvironment model. The tumor cells and immune cells were labeled by different fluorescent proteins. By using of skin-fold window chamber implanted into mice and intravital imaging technology, we could dynamically observe the different immune cells in tumor microenvironment. After data analysis from the video, we could know the behavior of TILs, DCs and Tregs in tumor immune microenvironment; furthermore, we could know these immune cells play different roles in the tumor microenvironment.

Citation Download Citation

Shuhong Qi and Zhihong Zhang "Intravital imaging of multicolor-labeled tumor immune microenvironment through skin-fold window chamber", Proc. SPIE 9324, Biophotonics and Immune Responses X, 93240F (9 March 2015); https://doi.org/10.1117/12.2078084

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KEYWORDS

Tumors

Fluorescent proteins

Signal detection

Biomedical optics

Microscopy

3D image processing

Data analysis

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