9 March 2015 Detection of squamous carcinoma cells using gold nanoparticles
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Proceedings Volume 9324, Biophotonics and Immune Responses X; 93240N (2015) https://doi.org/10.1117/12.2081783
Event: SPIE BiOS, 2015, San Francisco, California, United States
The goal of this study is to use gold nanoparticle as a diagnostic agent to detect human squamous carcinoma cells. Gold nanoparticles were synthesized and the gold nanoparticle size was 34.3 ± 6.2 nm. Based on the over-expression of epidermal growth factor receptor (EGFR) biomarkers in squamous carcinoma cells, we hypothesized that EGFR could be a feasible biomarker with a target moiety for detection. We further modified polyclonal antibodies of EGFR on the surface of gold nanoparticles. We found selected squamous carcinoma cells can be selectively detected using EGFR antibody-modified gold nanoparticles via receptor-mediated endocytosis. Cell death was also examined to determine the survival status of squamous carcinoma cells with respect to gold nanoparticle treatment and EGFR polyclonal antibody modification.
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Wei-Yun Dai, Wei-Yun Dai, Sze-tsen Lee, Sze-tsen Lee, Yih-Chih Hsu, Yih-Chih Hsu, "Detection of squamous carcinoma cells using gold nanoparticles", Proc. SPIE 9324, Biophotonics and Immune Responses X, 93240N (9 March 2015); doi: 10.1117/12.2081783; https://doi.org/10.1117/12.2081783

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