Real-time monitoring of tumor response to preoperative radiochemotherapy for rectal carcinoma by nonlinear optical microscopy

Lianhuang Li; Zhifen Chen; Xingfu Wang; Weizhong Jiang; Guoxian Guan; Jianxin Chen

doi:10.1117/12.2078055

9 March 2015 Real-time monitoring of tumor response to preoperative radiochemotherapy for rectal carcinoma by nonlinear optical microscopy

Lianhuang Li, Zhifen Chen, Xingfu Wang, Weizhong Jiang, Guoxian Guan, Jianxin Chen

Author Affiliations +

Proceedings Volume 9324, Biophotonics and Immune Responses X; 93240R (2015) https://doi.org/10.1117/12.2078055
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The continuing advancement of nonlinear optical imaging techniques has opened many new windows in biological exploration. In this work, the nonlinear optical microscopy, based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG), was extended to probe tumor response to preoperative radiochemotherapy (RCT) for rectal carcinoma. It was found that MPM has the ability of direct visualization of histopathologic changes in rectal carcinoma following preoperative RCT including stromal fibrosis, colloid response and residual tumors. Our results also showed the capability of MPM using the quantitative analyses of images to quantify these changes. This work may provide the groundwork for further exploration into the application of multiphoton-based endoscopy in a clinical setting.

Citation Download Citation

Lianhuang Li, Zhifen Chen, Xingfu Wang, Weizhong Jiang, Guoxian Guan, and Jianxin Chen "Real-time monitoring of tumor response to preoperative radiochemotherapy for rectal carcinoma by nonlinear optical microscopy", Proc. SPIE 9324, Biophotonics and Immune Responses X, 93240R (9 March 2015); https://doi.org/10.1117/12.2078055

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