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6 March 2015 Fast low-noise Brillouin spectroscopy measurements of elasticity for corneal crosslinking
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The Brillouin scattering spectra of biological systems have shown to be inherently related to the intrinsic elasticity and molecular constants of tissues involved. Our approach of combining confocal microscopy and high-resolution Brillouin spectroscopy via a virtual imaging phase array enabled 10-microsecond single-pixel acquisition time without dedicated spatial filtering. Such an approach is adapted via a single-frequency fiber-coupled 780-nm wavelength laser, frequency stabilized by Rb-D2 absorption line, polarization extinction scheme, ASE filtering, heated Rb-vapor Rayleigh-scattering absorbent, and spectroscopic EMCCD camera, unified as CMS-VIPA: confocal virtual-imaging phase array microscopespectrometer. Steady strengthening of corneal bulk modulus was observed via spectral shifts of Brillouin scattering from 5.0-5.2 GHz in untreated porcine eyes to 5.7-5.9 GHz in ones cross-linked in riboflavin plus UV-A light  at 0.7-0.9 GHz level of enhancement. The cross-linking depths reaching 300400 microns were measured, as predicted by modeling. A noncontact Brillouin spectroscopic microscopy system for in-vivo corneal elasticity measurement is under development.
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Michael Bukshtab, Amit Paranjape, Marc Friedman, and David Muller "Fast low-noise Brillouin spectroscopy measurements of elasticity for corneal crosslinking", Proc. SPIE 9327, Optical Elastography and Tissue Biomechanics II, 932718 (6 March 2015);

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