2 March 2015 Optical studies of oxidative stress in pulmonary artery endothelial cells
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Abstract
Reactive oxygen species (ROS) play an essential role in facilitating signal transduction processes within the cell and modulating the injuries. However, the generation of ROS is tightly controlled both spatially and temporally within the cell, making the study of ROS dynamics particularly difficult. This study present a novel protocol to quantify the dynamic of the mitochondrial superoxide as a precursor of reactive oxygen species. To regulate the mitochondrial superoxide level, metabolic perturbation was induced by administration of potassium cyanide (KCN). The presented method was able to monitor and measure the superoxide production rate over time. Our results demonstrated that the metabolic inhibitor, potassium cyanide (KCN) induced a significant increase in the rate of superoxide production in mitochondria of fetal pulmonary artery endothelial cells (FPAEC). Presented method sets the stage to study different ROS mediated injuries in vitro.
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Zahra Ghanian, Reyhaneh Sepehr, Annie Eis, Ganesh Kondouri, Mahsa Ranji, "Optical studies of oxidative stress in pulmonary artery endothelial cells", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 932807 (2 March 2015); doi: 10.1117/12.2076658; https://doi.org/10.1117/12.2076658
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