2 March 2015 Human oral mucosal epithelial cell sheets imaging with high-resolution phase-diversity homodyne OCT
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Abstract
There is a need for development of non-invasive technique to evaluate regenerative tissues such as cell sheets for transplantation. We demonstrated non-invasive imaging inside living cell sheets of human oral mucosal epithelial cells by phase-diversity homodyne optical coherence tomography (OCT). The new method OCT developed in Hitachi enables cell imaging because of high resolution (axial resolution; ~2.6 μm, lateral resolution; ~1 μm, in the air). Nuclei inside cell sheets were imaged with sufficient spatial resolution to identify each cell. It suggested that the new method OCT could be useful for non-invasive cell sheet evaluation test.
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Naoko Senda, Kentaro Osawa, "Human oral mucosal epithelial cell sheets imaging with high-resolution phase-diversity homodyne OCT", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93281E (2 March 2015); doi: 10.1117/12.2076762; https://doi.org/10.1117/12.2076762
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