5 March 2015 Flavin fluorescence lifetime imaging of living peripheral blood mononuclear cells on micro and nano-structured surfaces
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Abstract
Fabricated micro- and nano-structured surfaces were evaluated for use with living cells. Metabolic state was tested by means of endogenous flavin fluorescence of living peripheral blood mononuclear cells (PBMC) positioned on a coverslip, non-covered, or covered with micro- or nano-structured surfaces (OrmoComp polymer structures produced by 2-photon photopolymerisation, or Zinc Oxide (ZnO) layer fabricated by pulsed laser deposition). Confocal microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) were employed to gather flavin fluorescence lifetime images of living PBMC on structured surfaces. Gathered data are the first step towards monitoring of the live cell interaction with different micro/nano-structured surfaces and thus evaluate their potential applicability in the biomedical field.
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Tibor Teplicky, J. Horilova, J. Bruncko, C. Gladine, I. Lajdova, A. Mateasik, D. Chorvat, A. Marcek Chorvatova, "Flavin fluorescence lifetime imaging of living peripheral blood mononuclear cells on micro and nano-structured surfaces", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93290D (5 March 2015); doi: 10.1117/12.2076706; https://doi.org/10.1117/12.2076706
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