The reprogramming of somatic cells into induced pluripotent stem (iPS) cells can be evoked through the ectopic expression of defined transcription factors. Conventional approaches utilize retro/lenti-viruses to deliver genes/transcription factors as well as to facilitate the integration of transcription factors into that of the host genome. However, the use of viruses may result in insertional mutations caused by the random integration of genes and as a result, this may limit the use within clinical applications due to the risk of the formation of cancer. In this study, a new approach is demonstrated in realizing non-viral reprogramming through the use of ultrashort laser pulses, to introduce transcription factors into the cell so as to generate iPS cells.
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Aisada Uchugonova, Hans Georg Breunig, Ana Batista, Karsten König, "Optical reprogramming with ultrashort femtosecond laser pulses," Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93290U (5 March 2015);