5 March 2015 Four-wave mixing microscopy: a high potential nonlinear imaging method
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In this work we present non-resonant four-wave mixing microscopy as an additional contrast mechanism in nonlinear microscopy. The setup for this technique was based on a commercially available multiphoton microscope setup equipped with a titanium:sapphire-laser and an optical parametric oscillator as light sources. Fundamental system characteristics with respect to the spatio-temporal pulse overlap and the influence of aberrations on the process are presented. Experiments regarding the directionality of the four-wave mixing signal performed on fresh porcine meat showed an average ratio of the backward to forward signal mean intensity of 0.16 ± 0.01. Nevertheless, structural information is comparable for both detection modalities. This highlights the potential of four-wave mixing microscopy for in vivo applications. Furthermore, results on porcine meat show the additional contrast generated by four-wave mixing. In summary, the results show a great potential of non-resonant four-wave mixing microscopy as label-free imaging modality in the biomedical sciences.
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Tobias Ehmke, Tobias Ehmke, Andreas Knebl, Andreas Knebl, Alexander Heisterkamp, Alexander Heisterkamp, "Four-wave mixing microscopy: a high potential nonlinear imaging method", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 932912 (5 March 2015); doi: 10.1117/12.2076743; https://doi.org/10.1117/12.2076743

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