5 March 2015 Histology in vivo: chemical contrast combined with clinical multimodal multiphoton tomography
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Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 93291N (2015); doi: 10.1117/12.2078307
Event: SPIE BiOS, 2015, San Francisco, California, United States
Abstract
Label-free multiphoton tomography based on two-photon autofluorescence, fluorescence lifetime, and second harmonic generation imaging can be supplemented by coherent anti-Stokes Raman scattering. We present a compact, mobile and flexible clinical tomograph equipped with a novel detector design with multiple miniaturized detectors for individual acquisition of all four contrast mechanisms. Imaging of endogenous fluorophores, SHG-active collagen as well as nonfluorescent lipids in human skin in vivo is possible with this clinical tomograph paving the way towards in vivo histology.
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Martin Weinigel, Hans Georg Breunig, Karsten Koenig, "Histology in vivo: chemical contrast combined with clinical multimodal multiphoton tomography", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93291N (5 March 2015); doi: 10.1117/12.2078307; https://doi.org/10.1117/12.2078307
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KEYWORDS
Skin

In vivo imaging

Multiphoton tomography

Fluorescence lifetime imaging

Atrial fibrillation

Head

Imaging systems

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