5 March 2015 Simultaneous quadruple modal nonlinear optical imaging for gastric diseases diagnosis and characterization
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We report the development of a unique simultaneous quadruple-modal nonlinear optical microscopy (i.e., stimulated Raman scattering (SRS), second-harmonic generation (SHG), two-photon excitation fluorescence (TPEF), and third-harmonic generation (THG)) platform for characterization of the gastric diseases (i.e., gastritis, intestinal metaplasia (IM), intestinal type adenocarcinoma). SRS highlights the goblet cells found in IM. SHG images the distribution of collagen in lamina propria. Collagen is found to aggregate for intestinal type adenocarcinoma. TPEF reveals the cell morphology and can reflect the damage inside glands caused by the diseases. THG visualizes the nuclei with high spatial resolution, which facilitates the identification of neutrophils that are usually used as a feature of inflammation. This work shows that the co-registration of quadruple-modal images can be an effective means for diagnosis and characterization of gastric diseases at the cellular and molecular levels.
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Zi Wang, Zi Wang, Wei Zheng, Wei Zheng, Jian Lin, Jian Lin, Zhiwei Huang, Zhiwei Huang, "Simultaneous quadruple modal nonlinear optical imaging for gastric diseases diagnosis and characterization", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93291P (5 March 2015); doi: 10.1117/12.2080901; https://doi.org/10.1117/12.2080901

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