A modular two-photon microscope for simultaneous imaging of distant cortical areas in vivo

Fabian F Voigt; Jerry L. Chen; Roland Krueppel; Fritjof Helmchen

doi:10.1117/12.2076388

5 March 2015 A modular two-photon microscope for simultaneous imaging of distant cortical areas in vivo

Fabian F Voigt, Jerry L. Chen, Roland Krueppel, Fritjof Helmchen

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 93292C (2015) https://doi.org/10.1117/12.2076388
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

We have designed and built a two-photon microscope which allows calcium imaging in awake, behaving animals across field-of-views (FOV) of up to 1.7 × 1.7 mm. A special scan system enables independent x,y, and z-positioning of two smaller sub-areas within this FOV for simultaneous functional recordings. This microscope enables us to optically record neuronal activity with cellular resolution across much larger spatial scales than previously possible and should help in deciphering the behavior-dependent flow of information within the neocortex. The microscope hard- and software are modular and can be extended to other imaging and photostimulation modalities.

Citation Download Citation

Fabian F Voigt, Jerry L. Chen, Roland Krueppel, and Fritjof Helmchen "A modular two-photon microscope for simultaneous imaging of distant cortical areas in vivo", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93292C (5 March 2015); https://doi.org/10.1117/12.2076388

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