Statistical analysis on activation and photo-bleaching of step-wise multi-photon activation fluorescence of melanin

Zetong Gu; Zhenhua Lai; Xi Zhang; Jihao Yin; Charles A. DiMarzio

doi:10.1117/12.2076991

5 March 2015 Statistical analysis on activation and photo-bleaching of step-wise multi-photon activation fluorescence of melanin

Zetong Gu, Zhenhua Lai, Xi Zhang, Jihao Yin, Charles A. DiMarzio

Author Affiliations +

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 93292I (2015) https://doi.org/10.1117/12.2076991
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Melanin is regarded as the most enigmatic pigments/biopolymers found in most organisms. We have shown previously that melanin goes through a step-wise multi-photon absorption process after the fluorescence has been activated with high laser intensity. No melanin step-wise multi-photon activation fluorescence (SMPAF) can be obtained without the activation process. The step-wise multi-photon activation fluorescence has been observed to require less laser power than what would be expected from a non–linear optical process. In this paper, we examined the power dependence of the activation process of melanin SMPAF at 830nm and 920nm wavelengths. We have conducted research using varying the laser power to activate the melanin in a point-scanning mode for multi-photon microscopy. We recorded the fluorescence signals and position. A sequence of experiments indicates the relationship of activation to power, energy and time so that we can optimize the power level. Also we explored regional analysis of melanin to study the spatial relationship in SMPAF and define three types of regions which exhibit differences in the activation process.

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Zetong Gu, Zhenhua Lai, Xi Zhang, Jihao Yin, and Charles A. DiMarzio "Statistical analysis on activation and photo-bleaching of step-wise multi-photon activation fluorescence of melanin", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93292I (5 March 2015); https://doi.org/10.1117/12.2076991

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KEYWORDS

Luminescence

Particles

Multiphoton processes

Camera shutters

Microscopes

Absorption

Statistical analysis

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