Multi-channel beam-scanning imaging at kHz frame rates by Lissajous trajectory microscopy

Justin A. Newman; Shane Z. Sullivan; Ryan D. Muir; Suhas Sreehari; Charles A. Bouman; Garth J. Simpson

doi:10.1117/12.2079212

9 March 2015 Multi-channel beam-scanning imaging at kHz frame rates by Lissajous trajectory microscopy

Justin A. Newman, Shane Z. Sullivan, Ryan D. Muir, Suhas Sreehari, Charles A. Bouman, Garth J. Simpson

Proceedings Volume 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII; 933009 (2015) https://doi.org/10.1117/12.2079212
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

A beam-scanning microscope based on Lissajous trajectory imaging is described for achieving streaming 2D imaging with continuous frame rates up to 1.4 kHz. The microscope utilizes two fast-scan resonant mirrors to direct the optical beam on a circuitous trajectory through the field of view. By separating the full Lissajous trajectory time-domain data into sub-trajectories (partial, undersampled trajectories) effective frame-rates much higher than the repeat time of the Lissajous trajectory are achieved with many unsampled pixels present. A model-based image reconstruction (MBIR) 3D in-painting algorithm is then used to interpolate the missing data for the unsampled pixels to recover full images. The MBIR algorithm uses a maximum a posteriori estimation with a generalized Gaussian Markov random field prior model for image interpolation. Because images are acquired using photomultiplier tubes or photodiodes, parallelization for multi-channel imaging is straightforward. Preliminary results show that when combined with the MBIR in-painting algorithm, this technique has the ability to generate kHz frame rate images across 6 total dimensions of space, time, and polarization for SHG, TPEF, and confocal reflective birefringence data on a multimodal imaging platform for biomedical imaging. The use of a multichannel data acquisition card allows for multimodal imaging with perfect image overlay. Image blur due to sample motion was also reduced by using higher frame rates.

Citation Download Citation

Justin A. Newman, Shane Z. Sullivan, Ryan D. Muir, Suhas Sreehari, Charles A. Bouman, and Garth J. Simpson "Multi-channel beam-scanning imaging at kHz frame rates by Lissajous trajectory microscopy", Proc. SPIE 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, 933009 (9 March 2015); https://doi.org/10.1117/12.2079212

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