A novel multimodal laser scanning microscope control system

Zhenhua Lai; Zetong Gu; Stephen Karasek; James McLean; Xi Zhang; Charles DiMarzio; Jihao Yin; Daxi Xiong

doi:10.1117/12.2076846

9 March 2015 A novel multimodal laser scanning microscope control system

Zhenhua Lai, Zetong Gu, Stephen Karasek, James McLean, Xi Zhang, Charles DiMarzio, Jihao Yin, Daxi Xiong

Proceedings Volume 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII; 93300D (2015) https://doi.org/10.1117/12.2076846
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Traditional laser scanning microscopes require complex control systems to synchronize and control image acquisition. The control system is especially cumbersome in the multimodal laser scanning microscope. We have developed a novel multimodal laser scanning microscope control system based on a National Instruments multifunction data acquisition device (DAQ), which serves as both a data acquisition device and a programmable signal generator. The novel control system is low-cost and easy-to-build, with all components off-the-shelf. We have applied the control system in a multimodal laser scanning microscope. The control system has not only significantly decreased the complexity of the microscope, but also increased the system flexibility. We have demonstrated that the system can be easily customized for various applications.

Citation Download Citation

Zhenhua Lai, Zetong Gu, Stephen Karasek, James McLean, Xi Zhang, Charles DiMarzio, Jihao Yin, and Daxi Xiong "A novel multimodal laser scanning microscope control system", Proc. SPIE 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, 93300D (9 March 2015); https://doi.org/10.1117/12.2076846

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KEYWORDS

Control systems

Laser scanners

Microscopes

Confocal microscopy

Data acquisition

Mirrors

Multiphoton fluorescence microscopy

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