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9 March 2015 H4 tail interactions revealed by fluorescent fluctuation spectroscopy
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Abstract
Post-translational modifications to histone tails nave been shown to play a large role in dictating the conformation of chromatin. Structural changes in chromatin can play a large role in gene expression as compact chromatin can occlude transcriptional machinery. The role of the flexible regions of H4 N terminal tails is investigated using fluorescent correlation spectroscopy and the photon counting histogram. The combination of these techniques allows for the distinction between intra-array and inter-array interactions, as well as reveals structural changes that result from these interactions. The H4 tail was found to partake in attractive intra-array interactions that compact the 6x167 nucleosome arrays but did not partake in inter-array interactions that lead to oligomerization.
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Nathan Nurse and Chongli Yuan "H4 tail interactions revealed by fluorescent fluctuation spectroscopy", Proc. SPIE 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII, 933104 (9 March 2015); https://doi.org/10.1117/12.2079810
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