Mapping DNA-Lac repressor interaction with ultra-fast optical tweezers

Carina Monico; Alessia Tempestini; Francesco Vanzi; Francesco S. Pavone; Marco Capitanio

doi:10.1117/12.2080347

9 March 2015 Mapping DNA-Lac repressor interaction with ultra-fast optical tweezers

Carina Monico, Alessia Tempestini, Francesco Vanzi, Francesco S. Pavone, Marco Capitanio

Proceedings Volume 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII; 933116 (2015) https://doi.org/10.1117/12.2080347
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The lac operon is a well-known example of gene expression regulation, based on the specific interaction of Lac repressor protein (LacI) with its target DNA sequence (operator). We recently developed an ultrafast force-clamp laser trap technique capable of probing molecular interactions with sub-ms temporal resolution, under controlled pN-range forces. With this technique, we tested the interaction of LacI with different DNA constructs. Based on position along the DNA sequence, the observed interactions can be interpreted as specific binding to operator sequences and transient interactions with nonspecific sequences.

Citation Download Citation

Carina Monico, Alessia Tempestini, Francesco Vanzi, Francesco S. Pavone, and Marco Capitanio "Mapping DNA-Lac repressor interaction with ultra-fast optical tweezers", Proc. SPIE 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII, 933116 (9 March 2015); https://doi.org/10.1117/12.2080347

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