Using SPIM to track the development of the focal power of the zebrafish lens

Laura K. Young; Miguel Jarrin; Christopher D. Saunter; Roy Quinlan; John M. Girkin

doi:10.1117/12.2079887

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10 March 2015 Using SPIM to track the development of the focal power of the zebrafish lens

Laura K. Young, Miguel Jarrin, Christopher D. Saunter, Roy Quinlan, John M. Girkin

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Proceedings Volume 9334, Optical Methods in Developmental Biology III; 933408 (2015) https://doi.org/10.1117/12.2079887
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Selective plane illumination microscopy (SPIM) is a 3D imaging technique that uses a sheet of light to optically section a sample in vivo. A cylindrical lens focuses collimated light in one dimension, producing a sheet that is formed in the sample via an objective lens. Any optical power within the sample will additionally refract the light sheet passing through it. We exploit this effect to track the development of the optical power of the zebrafish lens over the first 4 days post fertilisation (dpf). We show that light is focussed on to the photoreceptor layer of the retina at 4 dpf.

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Laura K. Young, Miguel Jarrin, Christopher D. Saunter, Roy Quinlan, and John M. Girkin "Using SPIM to track the development of the focal power of the zebrafish lens", Proc. SPIE 9334, Optical Methods in Developmental Biology III, 933408 (10 March 2015); https://doi.org/10.1117/12.2079887

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