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31 March 2015 Realtime wavefront sensing in a SPIM microscope, and active aberration tracking
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Abstract
Adaptive optics (AO) can potentially allow high resolution imaging deep inside living tissue, mitigating against the loss of resolution due to aberrations caused by overlying tissue. Closed-loop AO correction is particularly attractive for moving tissue and spatially varying aberrations, but this requires direct wavefront sensing, which in turn requires suitable "guide stars" for use as wavefront references. We present a novel method for generating an orthogonally illuminated guide star suitable for direct wavefront sensing in a wide range of fluorescent biological structures, along with results demonstrating its use for measuring time-varying aberrations, in vivo.
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Jonathan M. Taylor, Christopher D. Saunter, Cyril Bourgenot, John M. Girkin, and Gordon D. Love "Realtime wavefront sensing in a SPIM microscope, and active aberration tracking", Proc. SPIE 9335, Adaptive Optics and Wavefront Control for Biological Systems, 93350A (31 March 2015); https://doi.org/10.1117/12.2080061
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