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11 March 2015 Phase correction in low coherence diffraction phase microscopy using the optical transfer function
Chris Edwards, Gabriel Popescu, Lynford L. Goddard
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Proceedings Volume 9336, Quantitative Phase Imaging; 933610 (2015) https://doi.org/10.1117/12.2080318
Event: SPIE BiOS, 2015, San Francisco, California, United States
Abstract
In this work, we experimentally determine the transfer function of our recently reported epi-illumination white light diffraction phase microscopy (epi-wDPM) system. The transfer function identifies how the low frequencies below k0NAcon are modified due to the limited spatial coherence and how the high frequencies above k0NAobj are affected due to the limited objective numerical aperture. Using this transfer function, we perform deconvolution to remove the halo and obtain proper quantitative phase measurements without the need for excessive spatial filtering. The wDPM and epi-wDPM systems are now capable of obtaining halo-free images with proper topography at much higher speeds.
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Chris Edwards, Gabriel Popescu, and Lynford L. Goddard "Phase correction in low coherence diffraction phase microscopy using the optical transfer function", Proc. SPIE 9336, Quantitative Phase Imaging, 933610 (11 March 2015); https://doi.org/10.1117/12.2080318
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KEYWORDS
Optical transfer functions
Microscopy
Diffraction
Spatial coherence
Coherence (optics)
Image acquisition
Deconvolution
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