11 March 2015 Nuclear dynamics in metastatic cells studied by quantitative phase imaging
Author Affiliations +
Proceedings Volume 9336, Quantitative Phase Imaging; 93361Q (2015) https://doi.org/10.1117/12.2183296
Event: SPIE BiOS, 2015, San Francisco, California, United States
We used a new quantitative high spatiotemporal resolution phase imaging tool to explore the nuclear structure and dynamics of individual cells. We used a novel analysis tool to quantify the diffusion outside and inside the nucleus of live cells. We also obtained information about the nuclear spatio temporal mass density in metastatic cells. The results indicate that in the cytoplasm, the intracellular transport is mainly active (direct, deterministic), while inside the nucleus it is both active and passive (diffusive, random). We calculated the standard deviation of velocities in active transport and the diffusion coefficient for passive transport.
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Silvia Ceballos, Silvia Ceballos, Mikhail Kandel, Mikhail Kandel, Shamira Sridharan, Shamira Sridharan, Freddy Monroy, Freddy Monroy, Gabriel Popescu, Gabriel Popescu, } "Nuclear dynamics in metastatic cells studied by quantitative phase imaging", Proc. SPIE 9336, Quantitative Phase Imaging, 93361Q (11 March 2015); doi: 10.1117/12.2183296; https://doi.org/10.1117/12.2183296

Back to Top