Efficient delivery of quantum dots in live cells by gold nanoparticle mediated photoporation

Ranhua Xiong; Freya Joris; Ine De Cock; Jo Demeester; Stefaan C. De Smedt; Andre G. Skirtach; Kevin Braeckmans

doi:10.1117/12.2075659

12 March 2015 Efficient delivery of quantum dots in live cells by gold nanoparticle mediated photoporation

Ranhua Xiong, Freya Joris, Ine De Cock, Jo Demeester, Stefaan C. De Smedt, Andre G. Skirtach, Kevin Braeckmans

Proceedings Volume 9338, Colloidal Nanoparticles for Biomedical Applications X; 93380X (2015) https://doi.org/10.1117/12.2075659
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

There is considerable interest in using Quantum Dots (QDs) as fluorescent probes such for cellular imaging due to unique advantages in comparison with conventional molecular dyes. However, cytosolic delivery of QDs into live cells remains a major challenge. Here we demonstrate highly efficient delivery of PEG-coated QDs into live cells by means of laser-induced vapour nanobubbles. Using this procedure we succeeded in high-throughput loading of ~80% of cells while maintaining a cell viability of ~85%.

Citation Download Citation

Ranhua Xiong, Freya Joris, Ine De Cock, Jo Demeester, Stefaan C. De Smedt, Andre G. Skirtach, and Kevin Braeckmans "Efficient delivery of quantum dots in live cells by gold nanoparticle mediated photoporation", Proc. SPIE 9338, Colloidal Nanoparticles for Biomedical Applications X, 93380X (12 March 2015); https://doi.org/10.1117/12.2075659

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