12 March 2015 A folic acid labelled carbon quantum dot-protoporphryin IX conjugate for use in folate receptor targeted two-photon excited photodynamic therapy
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Abstract
Folic acid (FA) has been used as a molecular targeting strategy to improve the specificity of a CQD-protoporphyrin IX (CQD-PPIX) conjugate to folate receptor positive (FR+) HeLa cells for use in two-photon excited Photodynamic Therapy (TPE-PDT). FA was covalently attached to the CQD-PPIX conjugate to form a FA-CQD-PPIX conjugate. The uptake of the FA-CQD-PPIX conjugate in FR+ HeLa cells was shown to be 7 times greater than the CQD-PPIX conjugate, while both conjugates showed a similar uptake in FR negative (FR-) HT-47 cells. TPE-PDT experiments, using HeLa cells as a target, revealed a 30% improved cytotoxicity for cells treated with the FA-CQD-PPIX conjugate and TPE compared to controls treated with the CQD-PPIX conjugate and TPE. Collectively, these results suggest the presence of FA can facilitate targeting of CQD-sensitiser conjugates to FR+ cells resulting in an improved PDT effect.
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Dean Nicholas, Dean Nicholas, Colin Fowley, Colin Fowley, Anthony P. McHale, Anthony P. McHale, Sukanta Kamila, Sukanta Kamila, Jason Sheng, Jason Sheng, Jordan Atchison, Jordan Atchison, John F. Callan, John F. Callan, } "A folic acid labelled carbon quantum dot-protoporphryin IX conjugate for use in folate receptor targeted two-photon excited photodynamic therapy", Proc. SPIE 9338, Colloidal Nanoparticles for Biomedical Applications X, 933813 (12 March 2015); doi: 10.1117/12.2084821; https://doi.org/10.1117/12.2084821
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