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12 March 2015 In vivo detection of cancer cells with immunoconjugated fluorescent probes by macro zoom microscopy and two-photon microscopy
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Abstract
We developed a near infrared fluorophore-conjugated anti–Carcinoembryonic antigen (CEA) antibody for mice bearing tumor of CEA expressing cells, and demonstrated in vivo optical imaging by macro zoom microscopy. In the result, tumors of CEA-expressing cancer cells were specifically detected in vivo. Furthermore, cancer-specific fluorescence images were acquired at subcellular level in vivo by two-photon microscopy. In preclinical applications, the lymph node micrometastasis was also successfully visualized by two-photon microscopy. These results suggest that two-photon excitation microscopy in combination with an immunoconjugated probe could be widely adapted to cancer detection in clinical settings.
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Shigehiro Koga, Yusuke Oshima, Atsuhiko Hikita, Koichi Sato, Motohira Yoshida, Yuji Yamamoto, Tadahiro Iimura, Yuji Watanabe, and Takeshi Imamura "In vivo detection of cancer cells with immunoconjugated fluorescent probes by macro zoom microscopy and two-photon microscopy", Proc. SPIE 9339, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII, 93390J (12 March 2015); doi: 10.1117/12.2079092; https://doi.org/10.1117/12.2079092
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