Sorting on the basis of deformability of single cells in a femtosecond laser fabricated optofluidic device

F. Bragheri; P. Paiè; Tie Yang; G. Nava; Rebeca Martìnez Vàzquez; Maira Di Tano; Manuela Veglione; P. Minzioni; Chiara Mondello; I. Cristiani; R. Osellame

doi:10.1117/12.2079291

9 March 2015 Sorting on the basis of deformability of single cells in a femtosecond laser fabricated optofluidic device

F. Bragheri, P. Paiè, Tie Yang, G. Nava, Rebeca Martìnez Vàzquez, Maira Di Tano, Manuela Veglione, P. Minzioni, Chiara Mondello, I. Cristiani, R. Osellame

Author Affiliations +

Proceedings Volume 9355, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XV; 93550G (2015) https://doi.org/10.1117/12.2079291
Event: SPIE LASE, 2015, San Francisco, California, United States

Abstract

Optical stretching is a powerful technique for the mechanical phenotyping of single suspended cells that exploits cell deformability as an inherent functional marker. Dual-beam optical trapping and stretching of cells is a recognized tool to investigate their viscoelastic properties. The optical stretcher has the ability to deform cells through optical forces without physical contact or bead attachment. In addition, it is the only method that can be combined with microfluidic delivery, allowing for the serial, high-throughput measurement of the optical deformability and the selective sorting of single specific cells. Femtosecond laser micromachining can fabricate in the same chip both the microfluidic channel and the optical waveguides, producing a monolithic device with a very precise alignment between the components and very low sensitivity to external perturbations. Femtosecond laser irradiation in a fused silica chip followed by chemical etching in hydrofluoric acid has been used to fabricate the microfluidic channels where the cells move by pressure-driven flow. With the same femtosecond laser source two optical waveguides, orthogonal to the microfluidic channel and opposing each other, have been written inside the chip. Here we present an optimized writing process that provides improved wall roughness of the micro-channels allowing high-quality imaging. In addition, we will show results on cell sorting on the basis of mechanical properties in the same device: the different deformability exhibited by metastatic and tumorigenic cells has been exploited to obtain a metastasis-cells enriched sample. The enrichment is verified by exploiting, after cells collection, fluorescence microscopy.

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F. Bragheri, P. Paiè, Tie Yang, G. Nava, Rebeca Martìnez Vàzquez, Maira Di Tano, Manuela Veglione, P. Minzioni, Chiara Mondello, I. Cristiani, and R. Osellame "Sorting on the basis of deformability of single cells in a femtosecond laser fabricated optofluidic device", Proc. SPIE 9355, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XV, 93550G (9 March 2015); https://doi.org/10.1117/12.2079291

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