20 March 2015 Automatic detection of endothelial cells in 3D angiogenic sprouts from experimental phase contrast images
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Proceedings Volume 9413, Medical Imaging 2015: Image Processing; 94132I (2015); doi: 10.1117/12.2081819
Event: SPIE Medical Imaging, 2015, Orlando, Florida, United States
Abstract
Cell migration studies in 3D environments become more popular, as cell behaviors in 3D are more similar to the behaviors of cells in a living organism (in vivo). We focus on the 3D angiogenic sprouting in microfluidic devices, where Endothelial Cells (ECs) burrow into the gel matrix and form solid lumen vessels. Phase contrast microscopy is used for long-term observation of the unlabeled ECs in the 3D microfluidic devices. Two template matching based approaches are proposed to automatically detect the unlabeled ECs in the angiogenic sprouts from the acquired experimental phase contrast images. Cell and non-cell templates are obtained from these phase contrast images as the training data. The first approach applies Partial Least Square Regression (PLSR) to find the discriminative features and their corresponding weight to distinguish cells and non-cells, whereas the second approach relies on Principal Component Analysis (PCA) to reduce the template feature dimension and Support Vector Machine (SVM) to find their corresponding weight. Through a sliding window manner, the cells in the test images are detected. We then validate the detection accuracy by comparing the results with the same images acquired with a confocal microscope after cells are fixed and their nuclei are stained. More accurate numerical results are obtained for approach I (PLSR) compared to approach II (PCA & SVM) for cell detection. Automatic cell detection will aid in the understanding of cell migration in 3D environment and in turn result in a better understanding of angiogenesis.
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MengMeng Wang, Lee-Ling Sharon Ong, Justin Dauwels, H. Harry Asada, "Automatic detection of endothelial cells in 3D angiogenic sprouts from experimental phase contrast images", Proc. SPIE 9413, Medical Imaging 2015: Image Processing, 94132I (20 March 2015); doi: 10.1117/12.2081819; http://dx.doi.org/10.1117/12.2081819
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KEYWORDS
Phase contrast

3D image processing

Confocal microscopy

Principal component analysis

Image processing

Environmental sensing

Microfluidics

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