Automatic choroid cells segmentation and counting based on approximate convexity and concavity of chain code in fluorescence microscopic image

Weihua Lu; Xinjian Chen; Weifang Zhu; Lei Yang; Zhaoyuan Cao; Haoyu Chen

doi:10.1117/12.2081950

19 March 2015 Automatic choroid cells segmentation and counting based on approximate convexity and concavity of chain code in fluorescence microscopic image

Weihua Lu, Xinjian Chen, Weifang Zhu, Lei Yang, Zhaoyuan Cao, Haoyu Chen

Author Affiliations +

Proceedings Volume 9420, Medical Imaging 2015: Digital Pathology; 942010 (2015) https://doi.org/10.1117/12.2081950
Event: SPIE Medical Imaging, 2015, Orlando, Florida, United States

Abstract

In this paper, we proposed a method based on the Freeman chain code to segment and count rhesus choroid-retinal vascular endothelial cells (RF/6A) automatically for fluorescence microscopy images. The proposed method consists of four main steps. First, a threshold filter and morphological transform were applied to reduce the noise. Second, the boundary information was used to generate the Freeman chain codes. Third, the concave points were found based on the relationship between the difference of the chain code and the curvature. Finally, cells segmentation and counting were completed based on the characteristics of the number of the concave points, the area and shape of the cells. The proposed method was tested on 100 fluorescence microscopic cell images, and the average true positive rate (TPR) is 98.13% and the average false positive rate (FPR) is 4.47%, respectively. The preliminary results showed the feasibility and efficiency of the proposed method.

Citation Download Citation

Weihua Lu, Xinjian Chen, Weifang Zhu, Lei Yang, Zhaoyuan Cao, and Haoyu Chen "Automatic choroid cells segmentation and counting based on approximate convexity and concavity of chain code in fluorescence microscopic image", Proc. SPIE 9420, Medical Imaging 2015: Digital Pathology, 942010 (19 March 2015); https://doi.org/10.1117/12.2081950

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Image segmentation

Luminescence

Image compression

Image filtering

Microscopy

Distributed interactive simulations

Computer programming

Show All Keywords

Keywords/Phrases

Search In:

Publication Years