Time-resolved fluorescence monitoring of cholesterol in peripheral blood mononuclear cells

Z. Martinakova; J. Horilova; I. Lajdova; A. Marcek Chorvatova

doi:10.1117/12.2074288

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5 December 2014 Time-resolved fluorescence monitoring of cholesterol in peripheral blood mononuclear cells

Z. Martinakova; J. Horilova; I. Lajdova; A. Marcek Chorvatova

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Proceedings Volume 9441, 19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics; 94411L (2014) https://doi.org/10.1117/12.2074288
Event: XIX Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 2014, Jelenia Gora, Poland

Abstract

Precise evaluation of intracellular cholesterol distribution is crucial for improving diagnostics of diseased states associated with cholesterol alteration. Time-resolved fluorescence techniques are tested for non-invasive investigation of cholesterol in living cells. Fluorescent probe NBD attached to cholesterol was employed to evaluate cholesterol distribution in peripheral blood mononuclear cells (PBMC) isolated from the human blood. Fluorescence Lifetime Imaging Microscopy (FLIM) was successfully applied to simultaneously monitor the spatial distribution and the timeresolved characteristics of the NBD-cholesterol fluorescence in PBMC. Gathered data are the first step in the development of a new perspective non-invasive diagnostic method for evaluation of cholesterol modifications in diseases associated with disorders of lipid metabolism.

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Z. Martinakova, J. Horilova, I. Lajdova, and A. Marcek Chorvatova "Time-resolved fluorescence monitoring of cholesterol in peripheral blood mononuclear cells", Proc. SPIE 9441, 19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 94411L (5 December 2014); https://doi.org/10.1117/12.2074288

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