19 March 2015 Comparison of cerebral microcirculation of alloxan diabetes and healthy mice using laser speckle contrast imaging
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Abstract
The study of blood microcirculation is one of the most important problems of the medicine. This paper presents results of experimental study of cerebral blood flow microcirculation in mice with alloxan-induced diabetes using Temporal Laser Speckle Imaging (TLSI). Additionally, a direct effect of glucose water solution (concentration 20% and 45%) on blood flow microcirculation was studied. In the research, 20 white laboratory mice weighing 20-30 g were used. The TLSI method allows one to investigate time dependent scattering from the objects with complex dynamics, since it possesses greater temporal resolution. Results show that in brain of animal diabetic group diameter of sagittal vein is increased and the speed of blood flow reduced relative to the control group. Topical application of 20%- or 45%-glucose solutions also causes increase of diameter of blood vessels and slows down blood circulation. The results obtained show that diabetes development causes changes in the cerebral microcirculatory system and TLSI techniques can be effectively used to quantify these alterations.
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Polina A. Timoshina, Polina A. Timoshina, Rui Shi, Rui Shi, Yang Zhang, Yang Zhang, Dan Zhu, Dan Zhu, Oxana V. Semyachkina-Glushkovskaya, Oxana V. Semyachkina-Glushkovskaya, Valery V. Tuchin, Valery V. Tuchin, Qingming Luo, Qingming Luo, } "Comparison of cerebral microcirculation of alloxan diabetes and healthy mice using laser speckle contrast imaging ", Proc. SPIE 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics, 94480B (19 March 2015); doi: 10.1117/12.2179988; https://doi.org/10.1117/12.2179988
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